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Objective To investigate the epidemiology of canine congenital and primary glaucoma in the cases presented to the University of Zurich, Vetsuisse Faculty (UZH) from 1995 to 2009. Methods Information was obtained from the computer database of patients examined by members of the UZH Ophthalmology Service, between January 1995 and August 2009. Congenital and primary glaucoma was diagnosed based on the age of onset, the lack of evidence of any antecedent eye conditions, and/or the presence and severity of iridocorneal angle defects. The data was evaluated for breed, gender and age at presentation. Results A total of 5984 dogs presented to the UZH Ophthalmology service between 1995 and 2009. Four dogs of different breed were diagnosed with congenital glaucoma and 123 dogs were diagnosed with primary glaucoma. For the primary glaucomas the overall male to female ratio (M:F) was 1:1.41 and the age of onset ranged from 0.12 to 18.3 years with a mean of 7.3 ± 3.6 years. Data suggested a predisposition for primary glaucoma in the Siberian Husky, Magyar Vizsla and Newfoundland from 2004 to 2009. Conclusion The report presents the epidemiology of canine congenital and primary glaucomas presented to the UZH from 1995 to 2009. A previous suspicion of predisposition for primary glaucoma in the Newfoundland dog (n = 6) and the Magyar Vizsla breed (n = 8) was confirmed. 相似文献
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栉江珧研究现状及开发利用前景 总被引：2，自引：0，他引：2
对广泛分布于温、热带近海海域的大型深水贝类栉江珧的分类地位和地理分布作了介绍，并就栉江珧栖息状况，对水温、盐度、pH、溶解氧等各种环境因子适应性的生态习性作了详细描述。论述了水温对栉江珧的生长、性腺周期发育的影响。对栉江珧人工繁育过程中的亲贝暂养、催产及幼体培育的技术和养殖现状进行了总结。简要概述了江珧科物种同工酶的研究进展，并对栉江珧资源保护及可持续利用和今后研究方向作了探讨。 相似文献
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本研究以前期获得的栉江珧(Atrina pectinata)转录组数据为基础,筛选获得10550条微卫星标记(SSR),检出率为8.2％,平均9.01 kb出现1个SSR位点.设计并合成120对SSR引物,筛选得到36对能够稳定扩增的引物,并利用已获得的36对SSR引物对青岛海区栉江珧进行了群体遗传多样性分析.结果显示,12对引物的扩增片段表现出多态性,共产生42个等位基因,平均每个位点产生3.5个等位基因,平均观测杂合度(Ho)、平均期望杂合度(He)和平均多态信息含量(PIC)分别为0.417、0.604和0.526,表明青岛海区栉江珧群体的遗传多样性较高.本研究获得的EST-SSR标记和群体遗传信息为栉江珧的种质资源保护提供了重要的参考资料. 相似文献
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栉江瑶(Atrina pectinata)为我国重要的海洋经济双壳贝类,近年来,其养殖生产活动备受养殖企业关注,为发展和优化其人工繁殖技术,本研究探究了人工海水中铵离子浓度和pH的变化对栉江瑶新鲜精子游泳运动的激活作用,定量描述了高度活化状态下精子曲线运动速率(VCL)、直线运动速率(VSL)、平均路径运动速率(VAP)和鞭毛摆动频率(BCF)的运动学特征,并对精子ATP含量、ATP酶与超氧化物歧化酶(SOD)活性进行了记录。结果显示,简单提高海水pH可略微提高栉江瑶精子运动率,但无法使精子进入高度活化状态;含有铵离子的碱化海水可有效激发精子游泳运动,3 mmol/L氨海水激活效果最佳。经3 mmol/L氨海水激活后,精子活力等级(MI)在21 min内一直保持在≥4的状态,在激活的前3 min内精子运动率都在80%以上,VCL>56 μm/s,VSL>17 μm/s,VAP>30 μm/s,BCF>6 Hz。精子ATP含量在激活5 min后降低至初始含量的30.29% [(128.80± 66.92) μmol/g prot],随后,无显著变化(P>0.05)。精子Na+-K+-ATP酶和Ca2+-Mg2+-ATP酶活性在运动过程中较为稳定,其中,Na+-K+-ATP酶活性较低[(0.62±0.03) U/mg prot],Ca2+-Mg2+-ATP酶活性较高[(6.08± 0.04) U/mg prot]。精子SOD活性在15 min内逐步降低至[(1.23±0.73) U/mg prot],随后维持稳定。本研究可为栉江瑶精子激活机制深入研究提供基础,助力发展栉江瑶人工繁育技术。 相似文献
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Pectinate ligament dysplasia (PLD) in dogs is a congenital ocular abnormality affecting the iridocorneal angle, the presence of which may be associated with adult-onset primary glaucoma. This study demonstrates a significant association between PLD and glaucoma in Flat Coated Retrievers, and also a higher prevalence of PLD in Flat Coated Retrievers compared with other breeds. It is shown that the association between PLD and glaucoma was robust and not due to a small increase in PLD with age. There was also a significant association between PLD in offspring and parents and, using the offspring–parents regression, the heritability of PLD was estimated as approximately 0.7. As glaucoma is significantly related to PLD, glaucoma may also be heritable. Thus, glaucoma can be controlled by gonioscopic examination and elimination of animals with a high degree of PLD from a breeding program. Breeding from only those animals with a moderate or low PLD value or score should limit the expected prevalence of glaucoma in the resulting population to < 0.2%. The preceding study describes in detail the PLD survey, gonioscopic technique and some initial results obtained prior to this epidemiological investigation. 相似文献
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栉江珧(Atrina pectinata)和近江牡蛎(Crassostrea rivularis)是我国重要的海产经济贝类,建立其精子冷冻保存技术及精子冷冻库,对种质资源的保护和遗传育种具有重要意义.本研究对其精子冷冻保存过程中冷冻剂种类和浓度的选择、冷冻程序、平衡时间、保存体积、精液稀释方式、保存时间等多个影响因素进行了比较筛选,并利用显微镜直接检测和曙红Y(eosin Y)染色检测结合的方式检验精子存活率.结果表明,二甲亚砜(dimethyl sulfoxide,DMSO)作为抗冻剂的保存效果要明显优于甘油(glycerol,Gly),终浓度为8％～10％的二甲亚砜保存效果最好,其栉江珧和近江牡蛎精子活率均可达60％以上,而甘油保护下精子活率小于40％.精液的稀释方式采用将抗冻液等分为3份,依次与精液混合,每次间隔8 min,整个过程约30 min;此种精液稀释方式与一次性混合方式相比,精子活率可提高约30％.稀释后精液需要在4℃下平衡15～25 min,平衡样品与直接冷冻样品相比,精子活率提高约50％.精液降温程序为在样品投入液氮前,于液氮面以上15 cm处停留5 min,5 cm处停留10 min,精子活率可达60％以上;而未经此降温程序直接投入液氮中的样品精子活率接近0.与此降温程序相对应的样品冷冻体积在1.0～1.4 mL范围内为宜.冷冻180 d内复苏的精子活率由90％左右(鲜精)下降至60％左右,180 d后精子活率下降至56％左右.研究结果为栉江珧和近江牡蛎精子超低温冷冻保存、双壳类种质保护和遗传育种提供了理论和技术依据. 相似文献
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A. Benitez‐Hernández S.P.L. Jiménez‐Bárcenas E.Y. Sánchez‐Gutiérrez J.C. Pérez‐Urbiola D. Tovar‐Ramírez E. Palacios R. Civera‐Cerecedo 《Aquaculture Nutrition》2018,24(1):562-570
A 60‐day feeding trial to determine the nutritional value of marine by‐product meals in diets for longfin yellowtail Seriola rivoliana juveniles (48.1 ± 0.6 g initial weight) was conducted. Five diets were evaluated: a reference diet (RD; 500 g kg?1 CP, 130 g kg?1 L), containing 500 g kg?1 of fish meal (FM); three experimental diets with 125 g kg?1 of shrimp head (SM), Catarina scallop viscera (CM) or Pen shell viscera (PM) meals; and one diet (SCP) containing 125 g kg?1 of each of the experimental meals, to partially replace FM. Survival was not significantly affected by any treatment. Individual weight gain per day was high for the PM (5.3 ± 0.51 g d?1) and SM (4.7 ± 0.32 g d?1) diets, being significantly higher than the RD (3.5 ± 0.23 g d?1) and the other treatments (<1.2 g d?1). Feed intake was high in PM and SM diets, and very low in SCP and CM diets. Biochemical and haematological parameters were similar among treatments RD, PM and SM, while fish fed CM and SCP exhibited lower levels of total protein, cholesterol, haematocrit and haemoglobin. The results indicate that SM or PM can be used to partially replace FM in diets for yellowtail juveniles. 相似文献
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